dylight 649 anti goat secondary antibodies (Vector Laboratories)
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Vector Laboratories
dylight 649 anti goat secondary antibodies
Dylight 649 Anti Goat Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/DI-1649-1%2E5/DyLight+649+Goat+Anti-Rabbit+IgG+Antibody/pmc12508231__12276_2025_1533_MOESM1_ESM-62-20-25
Average 94 stars, based on 31 article reviews
Dylight 649 Anti Goat Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/DI-1649-1%2E5/DyLight+649+Goat+Anti-Rabbit+IgG+Antibody/pmc12508231__12276_2025_1533_MOESM1_ESM-62-20-25
Average 94 stars, based on 31 article reviews
dylight 649 anti goat secondary antibodies - by Bioz Stars,
2026-11
94/100 stars
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Incubation:Article Title: Decreased neuronal and increased endothelial fractalkine expression are associated with neuroinflammation in Parkinson’s disease and related disorders Article Snippet: After background staining was blocked for 1 h, sections were incubated in the first primary polyclonal antibody (CX3CL1, 1:1,000) or monoclonal antibody (TMEM119, 1:1,000) overnight separately, followed by biotinylated goat anti-rabbit antibody (BA1000, 1:200, Vector Lab) or biotinylated horse anti-mouse antibody (BA2000, 1:200, Vector Lab) for 1 h, and the Elite avidin–biotin complex (1:500; PK-6100, Vector) for 1 h. The immunofluorescent labeling was completed with 1:1,000 fluorescein tyramide (6456, TOCRIS) and 0.005% H 2 O 2 . .. After blockade for 1 h, the sections were then incubated in the second primary antibodies: phosphorylated α-syn monoclonal antibody (p-S129, 1:1000; ab51253/Abcam), α-syn oligomer-specific monoclonal antibody (Syn05, 1:1000; AS132718, Agrisera), anti-phospho-tau monoclonal antibody (AT8, 1:1,000; MN1020, ThermoFisher), or CD34 (1:10,000; 60180-2-Ig, proteintech) overnight followed by goat anti-rabbit secondary coupled to Article Title: Longitudinal Imaging of Injured Spinal Cord Myelin and White Matter with 3D Ultrashort Echo Time Magnetization Transfer (UTE-MT) and Diffusion MRI Article Snippet: .. Sections were permeabilized and blocked with PBS containing 0.3% Triton X-100 and 5% donkey serum for 2 h; then incubated with mouse anti-NF200 (Millipore Sigma, Burlington, MA, USA, MAB5262, 1 μg/mL) and rabbit anti-MBP (Cell Signaling, Danvers, MA, USA, 78896, 1:1000) in PBS containing 0.1% triton X-100 and 5% donkey serum at room temperature overnight; washed for 1 h; then incubated with cy3-conjugated anti-mouse (Jackson ImmunoResearch, West Grove, PA, USA, Code 715-167-003, 1 μg/mL), Article Title: Cholecystokinin input from the anterior cingulate cortex to the lateral periaqueductal gray mediates nocebo pain behavior in mice Article Snippet: In the retrograde tracing experiment, a monoclonal NeuN antibody (1:2,000 overnight, EMD Millipore MABN140, Darmstadt, Germany) or a polyclonal EMX1 antibody (1:50 overnight, Invitrogen, PA5-35373, Carlsbad, CA), were used as general or excitatory neuronal markers, respectively. .. Following primary antibody incubation, sections were washed three times for 5 min in Article Title: Stress-sensitive neural circuits change the gut microbiome via duodenal glands. Article Snippet: 3-4 images were obtained per sample. imageJ was used to measure mucin thickness across 30-40 different regions of each image, and then to calculate mean thickness values. .. Immunohistochemical staining To visualize Fos via immunofluorescence, sections were incubated with a Rabbit Anti-c-Fos antibody (Rabbit Anti-c-Fos ab190289, 1:1000), followed by Article Title: Loss of PCAF in proximal tubular cells exacerbates renal fibrosis by promoting partial epithelial-to-mesenchymal transition Article Snippet: After blocking, the cells were incubated overnight at 4°C with primary antibodies targeting PCAF (Santa Cruz, sc13124), ZO-1 (invitrogen, 61-7300), LC3 (Santa Cruz, sc398822), and vimentin (Santa Cruz, sc7557). .. Following primary antibody incubation, the slides were washed with PBS and incubated with Dylight 488 anti-mouse, Dylight 549 anti-rabbit, and Article Title: Stress-sensitive neural circuits change the gut microbiome via duodenal glands. Article Snippet: To visualize Brunner’s gland DMV innervation via immunofluorescence, 10mmBrunner’s gland slices were separated incubated at 4 C overnight with Goat Anti-GFP antibody (FITC) (Abcam, #ab6662, 1:1000) for DMV fiber; Rabbit polyclonal mCherry antibody (Abcam, #ab167453, 1:1000) for DMV synaptophysin; Goat-anti-ChAT antibody (Millipore, #AB144, 1:1000) for cholinergic neurons and Rabbit polyclonal to beta III Tubulin (Abcam, #ab18207, 1:1000) for neuron. .. 2nd antibodies were incubated at room temperature with Article Title: Stress-sensitive neural circuits change the gut microbiome via duodenal glands. Article Snippet: To visualize B cells, cell proliferation, and FDC light zones via immunofluorescence, 10mm spleen sections were incubated with Alexa647-conjugated anti-mouse IgD antibody (Biolegend, #405707, 1:200, Clone: 11-26c.2a), Brilliant Violet 421-conjuncted anti-mouse CD21/CD35 antibody (Biolegend, # 123421, 1:200, Clone: 7E9), and Alexa488-conjuncted anti-mouse/human Ki-67 antibody (Biolegend, # 151204, 1:200, Clone: 11F6) at 4 C overnight. .. Slices were washed three times using KPBS solution and imaged using Zeiss LSM980 Airyscan 2 with a 10X objective. ll OPEN ACCESS e14 Cell 187, 1–20.e1–e18, September 19, 2024 Please cite this article in press as: Chang et al., Stress-sensitive neural circuits change the gut microbiome via duodenal glands, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.019 Article To visualize goblet cells via immunofluorescence, 10mm intestine slices were incubated with Rabbit Anti-MUC2 antibody (Abcam, #ab272692, 1:1000), followed by Staining:Article Title: Decreased neuronal and increased endothelial fractalkine expression are associated with neuroinflammation in Parkinson’s disease and related disorders Article Snippet: After background staining was blocked for 1 h, sections were incubated in the first primary polyclonal antibody (CX3CL1, 1:1,000) or monoclonal antibody (TMEM119, 1:1,000) overnight separately, followed by biotinylated goat anti-rabbit antibody (BA1000, 1:200, Vector Lab) or biotinylated horse anti-mouse antibody (BA2000, 1:200, Vector Lab) for 1 h, and the Elite avidin–biotin complex (1:500; PK-6100, Vector) for 1 h. The immunofluorescent labeling was completed with 1:1,000 fluorescein tyramide (6456, TOCRIS) and 0.005% H 2 O 2 . .. After blockade for 1 h, the sections were then incubated in the second primary antibodies: phosphorylated α-syn monoclonal antibody (p-S129, 1:1000; ab51253/Abcam), α-syn oligomer-specific monoclonal antibody (Syn05, 1:1000; AS132718, Agrisera), anti-phospho-tau monoclonal antibody (AT8, 1:1,000; MN1020, ThermoFisher), or CD34 (1:10,000; 60180-2-Ig, proteintech) overnight followed by goat anti-rabbit secondary coupled to Article Title: Stress-sensitive neural circuits change the gut microbiome via duodenal glands. Article Snippet: 3-4 images were obtained per sample. imageJ was used to measure mucin thickness across 30-40 different regions of each image, and then to calculate mean thickness values. .. Immunohistochemical staining To visualize Fos via immunofluorescence, sections were incubated with a Rabbit Anti-c-Fos antibody (Rabbit Anti-c-Fos ab190289, 1:1000), followed by Immunohistochemical staining:Article Title: Stress-sensitive neural circuits change the gut microbiome via duodenal glands. Article Snippet: 3-4 images were obtained per sample. imageJ was used to measure mucin thickness across 30-40 different regions of each image, and then to calculate mean thickness values. .. Immunohistochemical staining To visualize Fos via immunofluorescence, sections were incubated with a Rabbit Anti-c-Fos antibody (Rabbit Anti-c-Fos ab190289, 1:1000), followed by Immunofluorescence:Article Title: Stress-sensitive neural circuits change the gut microbiome via duodenal glands. Article Snippet: 3-4 images were obtained per sample. imageJ was used to measure mucin thickness across 30-40 different regions of each image, and then to calculate mean thickness values. .. Immunohistochemical staining To visualize Fos via immunofluorescence, sections were incubated with a Rabbit Anti-c-Fos antibody (Rabbit Anti-c-Fos ab190289, 1:1000), followed by Article Title: Stress-sensitive neural circuits change the gut microbiome via duodenal glands. Article Snippet: To visualize B cells, cell proliferation, and FDC light zones via immunofluorescence, 10mm spleen sections were incubated with Alexa647-conjugated anti-mouse IgD antibody (Biolegend, #405707, 1:200, Clone: 11-26c.2a), Brilliant Violet 421-conjuncted anti-mouse CD21/CD35 antibody (Biolegend, # 123421, 1:200, Clone: 7E9), and Alexa488-conjuncted anti-mouse/human Ki-67 antibody (Biolegend, # 151204, 1:200, Clone: 11F6) at 4 C overnight. .. Slices were washed three times using KPBS solution and imaged using Zeiss LSM980 Airyscan 2 with a 10X objective. ll OPEN ACCESS e14 Cell 187, 1–20.e1–e18, September 19, 2024 Please cite this article in press as: Chang et al., Stress-sensitive neural circuits change the gut microbiome via duodenal glands, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.019 Article To visualize goblet cells via immunofluorescence, 10mm intestine slices were incubated with Rabbit Anti-MUC2 antibody (Abcam, #ab272692, 1:1000), followed by |